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Journal articles
Plant N-glycan profiling of minute amounts of material
Martial Séveno
1, 2
Gleysin Cabrera
3
Ada Triguero
3
Carole Burel
1
Jérôme Leprince
4
Christophe Rihouey
5
Louis-Philippe Vezina
2
Marc-André d'Aoust
2
Pauline Rudd
6
L. Royle
7
Raymond Dwek
7
David Harvey
8
Patrice Lerouge
1
José Cremata
3
Muriel Bardor
1
Martial Séveno 1, 2
Author IdHAL : martial-seveno ORCID : https://orcid.org/0000-0002-5000-8735
Gleysin Cabrera 3
Author
Ada Triguero 3
Author
Carole Burel 1
Author
Jérôme Leprince 4
Author IdHAL : jerome-leprince ORCID : https://orcid.org/0000-0002-7814-9927
Christophe Rihouey 5
Author
Louis-Philippe Vezina 2
Author
Marc-André d'Aoust 2
Author
Patrice Lerouge 1
Author
José Cremata 3
Author
Muriel Bardor 1
Author IdHAL : muriel-bardor ORCID : https://orcid.org/0000-0002-0966-903X
1
GTCV - Glycobiologie et transports chez les végétaux (bat. Biologie (extension) Place Emile Blondel 76821 MONT ST AIGNAN CEDEX - France)
- UNIROUEN - Université de Rouen Normandie (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR6037 (France)
2
Medicago Inc. (1020, Route de l'Église, bureau 600 - Québec, Québec G1V 3V9
- Canada)
3
CIGB - Center for Genetic Engineering and Biotechnology [Cuba] (Ave 31 between 158 y 190, Playa, P.O. Box 6162, Havana 10600, Cuba - Cuba)
4
Neuroendocrinologie cellulaire et moléculaire (Universite de Rouen 76821 MONT-SAINT-AIGNAN CEDEX - France)
- UNIROUEN - Université de Rouen Normandie : U413 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U413 (101, rue de Tolbiac, 75013 Paris - France)
5
PBS - Polymères Biopolymères Surfaces (Bd Maurice de Broglie F-76821 Mont Saint Aignan Cedex
Avenue de l'université, BP08, 76801 Saint Etienne du Rouvray Cedex - France)
- CNRS - Centre National de la Recherche Scientifique : UMR 6270 (France)
- UNIROUEN - Université de Rouen Normandie (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSA Rouen Normandie - Institut national des sciences appliquées Rouen Normandie (Avenue de l’Université 76801 Saint-Étienne-du-Rouvray Cedex - France)
- INSA - Institut National des Sciences Appliquées (France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INC3M - Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (Rue Tesnière
76821 MONT ST AIGNAN CEDEX - France)
- INC - Institut de Chimie du CNRS (3 Rue Michel-Ange 75 016 Paris - France)
- ENSICAEN - École Nationale Supérieure d'Ingénieurs de Caen (6, Boulevard du Maréchal Juin CS 45053 14050 CAEN cedex 04 - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSA Rouen Normandie - Institut national des sciences appliquées Rouen Normandie (Avenue de l’Université 76801 Saint-Étienne-du-Rouvray Cedex - France)
- INSA - Institut National des Sciences Appliquées (France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- ULH - Université Le Havre Normandie (25 rue Philippe Lebon - BP 1123 - 76063 Le Havre Cedex France - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- UNIROUEN - Université de Rouen Normandie (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- CNRS - Centre National de la Recherche Scientifique : FR3038 (France)
- UNICAEN - Université de Caen Normandie (Esplanade de la Paix - CS 14032 - 14032 CAEN Cedex 5 - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
6
UCD - University College Dublin [Dublin] (Belfield, Dublin 4 - Ireland)
7
Oxford Glycobiology Institute (University of Oxford South Parks Road OXFORD OXI 3QU - United Kingdom)
- University of Oxford [Oxford] (Wellington Square, Oxford OX1 2JD - United Kingdom)
8
UNSW - School of Mathematics and Statistics [Sydney] (University of New South Wales Sydney NSW 2052 Australia - Australia)
- UNSW - University of New South Wales [Sydney] (High St Kensington, Sydney, NSW 2052 - Australia)
Abstract : Development of convenient strategies for identification of plant N-glycan profiles has been driven by the emergence of plants as an expression system for therapeutic proteins. In this article, we reinvestigated qualitative and quantitative aspects of plant N-glycan profiling. The extraction of plant proteins through a phenol/ammonium acetate procedure followed by deglycosylation with peptide N-glycosidase A (PNGase A) and coupling to 2-aminobenzamide provides an oligosaccharide preparation containing reduced amounts of contaminants from plant cell wall polysaccharides. Such a preparation was also suitable for accurate qualitative and quantitative evaluation of the N-glycan content by mass spectrometry. Combining these approaches allows the profiling to be carried out from as low as 500 mg of fresh leaf material. We also demonstrated that collision-induced dissociation (CID) mass spectrometry in negative mode of N-glycans harboring alpha(1,3)- or alpha(1,6)-fucose residue on the proximal GlcNAc leads to specific fragmentation patterns, thereby allowing the discrimination of plant N-glycans from those arising from mammalian contamination.
Document type :
Journal articles
Complete list of metadata
https://hal-normandie-univ.archives-ouvertes.fr/hal-01974002
Contributor : Jérôme Leprince Connect in order to contact the contributor
Submitted on : Tuesday, January 8, 2019 - 3:19:02 PM
Last modification on : Thursday, December 2, 2021 - 3:14:51 AM
Contributor : Jérôme Leprince Connect in order to contact the contributor
Submitted on : Tuesday, January 8, 2019 - 3:19:02 PM
Last modification on : Thursday, December 2, 2021 - 3:14:51 AM
Identifiers
- HAL Id : hal-01974002, version 1
- DOI : 10.1016/j.ab.2008.04.034
- PUBMED : 18482571
Collections
COMUE-NORMANDIE | CNRS | INSA-ROUEN | UNIROUEN | INSA-GROUPE | PBS | ENSICAEN | UNILEHAVRE | UNICAEN | INC-CNRS
Citation
Martial Séveno, Gleysin Cabrera, Ada Triguero, Carole Burel, Jérôme Leprince, et al.. Plant N-glycan profiling of minute amounts of material. Analytical Biochemistry, Elsevier Masson, 2008, 379 (1), pp.66-72. ⟨10.1016/j.ab.2008.04.034⟩. ⟨hal-01974002⟩
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