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Journal articles
PACAP Stimulates Biosynthesis and Release of Endozepines from Rat Astrocytes
Olfa Masmoudi-Kouki
1, 2, 3
Pierrick Gandolfo
2, 3
Jérôme Leprince
2, 3
David Vaudry
2, 3
Georges Pelletier
4
Alain Fournier
5
Hubert Vaudry
2, 3
Marie-Christine Tonon
2, 3
Olfa Masmoudi-Kouki 1, 2, 3
Author
Pierrick Gandolfo 2, 3
Author
Jérôme Leprince 2, 3
Author IdHAL : jerome-leprince ORCID : https://orcid.org/0000-0002-7814-9927
David Vaudry 2, 3
Author IdHAL : david-vaudry ORCID : https://orcid.org/0000-0003-3567-7452
Georges Pelletier 4
Author
Alain Fournier 5
Author
Hubert Vaudry 2, 3
Author
Marie-Christine Tonon 2, 3
Author
1
University of Tunis - Faculty of Sciences of Tunis (University of Tunis El Mana 2060 Tunis - Tunisia)
- UTM - Université de Tunis El Manar (Campus Universitaire Farhat Hached B.P. n° 94 - ROMMANA 1068 Tunis - Tunisia)
2
Neuroendocrinologie cellulaire et moléculaire (Universite de Rouen 76821 MONT-SAINT-AIGNAN CEDEX - France)
- UNIROUEN - Université de Rouen Normandie : U413 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U413 (101, rue de Tolbiac, 75013 Paris - France)
3
IFRMP 23 - Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (Place Emile Blondel 76821 Mont Saint Aignan cedex - France)
- CHU Rouen (1, rue de Germont 76031 Rouen cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- ULH - Université Le Havre Normandie (25 rue Philippe Lebon - BP 1123 - 76063 Le Havre Cedex France - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- UNIROUEN - Université de Rouen Normandie (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- CRLCC Henri Becquerel (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : IFR23 (101, rue de Tolbiac, 75013 Paris - France)
- CNRS - Centre National de la Recherche Scientifique : IFR23 (France)
4
Laval University Medical center (Canada)
- ULaval - Université Laval [Québec] (2325, rue de l'Université Québec G1V 0A6 - Canada)
5
INRS-IAF - Institut Armand Frappier (531 boul. des Prairies Laval (Québec) H7V 1B7 - Canada)
- INRS - Institut National de la Recherche Scientifique [Québec] (490 rue de la Couronne, Québec, QC G1K 9A9 - Canada)
- RIIP - Réseau International des Instituts Pasteur (25, rue du Dr Roux 75724 Paris Cedex 15 - France)
Abstract : Astrocytes synthesize and release endozepines, a family of neuropeptides related to diazepam-binding inhibitor (DBI). Astroglial cells also express the receptors of pituitary adenylate cyclase-activating polypeptide (PACAP) and vasoactive intestinal polypeptide (VIP). In the present article, we show that PACAP dose dependently increases DBI gene expression and stimulates endozepine release through activation of PAC1-R. PACAP increases cAMP formation, enhances polyphosphoinositide turnover, and evokes calcium mobilization from intracellular Ca2+ pools. The effect of PACAP on endozepine release is mediated through the adenylyl cyclase/PKA pathway while the downregulation of astrocyte response to PACAP can be ascribed to activation of the PLC/PKC pathway.
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Journal articles
Complete list of metadatas
https://hal-normandie-univ.archives-ouvertes.fr/hal-01973027
Contributor : Jérôme Leprince <>
Submitted on : Tuesday, January 8, 2019 - 10:06:28 AM
Last modification on : Wednesday, October 28, 2020 - 9:52:05 AM
Contributor : Jérôme Leprince <>
Submitted on : Tuesday, January 8, 2019 - 10:06:28 AM
Last modification on : Wednesday, October 28, 2020 - 9:52:05 AM
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Identifiers
- HAL Id : hal-01973027, version 1
- DOI : 10.1196/annals.1317.094
- PUBMED : 16888201
Collections
COMUE-NORMANDIE | UNIROUEN | CNRS | RIIP | INRS-IAF | FNCLCC | BECQUEREL | UNILEHAVRE
Citation
Olfa Masmoudi-Kouki, Pierrick Gandolfo, Jérôme Leprince, David Vaudry, Georges Pelletier, et al.. PACAP Stimulates Biosynthesis and Release of Endozepines from Rat Astrocytes. Annals of the New York Academy of Sciences, Wiley, 2006, 1070 (1), pp.411-416. ⟨10.1196/annals.1317.094⟩. ⟨hal-01973027⟩
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