Urotensin II and urotensin II-related peptide activate somatostatin receptor subtypes 2 and 5

Maria Malagon (1, 2) , Marcelo Molina (1) , Manuel Gahete (1, 2) , Mario Duran-Prado (1) , Antonio Martinez-Fuentes (1, 2) , Francisco Alcain (1) , Marie-Christine Tonon (3, 4) , Jérôme Leprince (3, 4) , Hubert Vaudry (3, 4) , Justo Castaño (1, 2) , Rafael Vazquez-Martinez (1, 2)

1 Universidad de Córdoba = University of Córdoba [Córdoba]
2 ISC - Instituto de Salud Carlos III [Madrid]
3 Neuroendocrinologie cellulaire et moléculaire
4 IFRMP 23 - Institut Fédératif de Recherches Multidisciplinaires sur les Peptides

The UII and urotensin II-related peptide (URP) genes belong to the same superfamily as the somatostatin gene. It has been previously shown that somatostatin activates the UII-receptor (UTR). In contrast, the possible interaction between UII and URP and somatostatin receptors has remained scarcely analyzed. Herein, we have investigated the effects of UII and URP on cell proliferation and free cytosolic Ca2+ concentration ([Ca2+]i) in CHO-K1 cells stably expressing the porcine somatostatin receptor subtypes sst2 and sst5. Results show that both UII and URP induce stimulation of cell proliferation mediated by sst2 receptors and UII provokes inhibition of cell proliferation mediated by sst5 receptors. UII and URP also provoked an increase of [Ca2+]i in both sst2- and sst5-transfected cells. Together, our present data demonstrate that UII and URP directly activate sst2 and sst5 and thus mimic the effect of somatostatin on its cognate receptors.

Domaines: Chimie thérapeutique; Interactions cellulaires [q-bio.CB]; Neurobiologie; Pharmacologie

Maria Malagon, Marcelo Molina, Manuel Gahete, Mario Duran-Prado, Antonio Martinez-Fuentes, et al.. Urotensin II and urotensin II-related peptide activate somatostatin receptor subtypes 2 and 5. Peptides, 2008, 29 (5), pp.711-720. ⟨10.1016/j.peptides.2007.12.015⟩. ⟨hal-01973000⟩