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Detection and quantitation of equid gammaherpesviruses (EHV-2, EHV-5) in nasal swabs using an accredited standardised quantitative PCR method

Abstract : Equid gammaherpesviruses-2 and -5 are involved in respiratory problems, with potential clinical man-ifestations such as nasal discharge, pharyngitis and swollen lymph nodes. These viruses are sometimesassociated with a poor-performance syndrome, which may result in a significant and negative economicimpact for the horse industry. The aim of the present study was to develop and validate quantitative PCRmethods for the detection and quantitation of EHV-2 and EHV-5 in equine respiratory fluids. Two dis-tinct tests were characterised: (a) for the qPCR alone and (b) for the whole method (extraction and qPCR)according to the standard model AFNOR XP U47-600-2 (viz., specificity, quantifiable sensibility, linearity,accuracy, range of application, trueness, precision, repeatability and precision of reproducibility). EHV-2and EHV-5 detection were performed on nasal swabs collected from 172 horses, all of which exhibitedclinical signs of respiratory disease. The data revealed a high rate of EHV-2/EHV-5 co-detection that wascorrelated significantly with age. Viral load of EHV-2 was significantly higher in young horses whereasviral load of EHV-5 was not significantly different with age.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01968342
Contributor : Erika Hue <>
Submitted on : Wednesday, January 2, 2019 - 3:25:56 PM
Last modification on : Friday, September 18, 2020 - 12:31:09 PM

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Erika Hue, Guillaume Fortier, Christine Fortier, Albertine Léon, Eric Richard, et al.. Detection and quantitation of equid gammaherpesviruses (EHV-2, EHV-5) in nasal swabs using an accredited standardised quantitative PCR method. Journal of Virological Methods, Elsevier, 2014, 198, pp.18-25. ⟨10.1016/j.jviromet.2013.12.008⟩. ⟨hal-01968342⟩

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