Purification and characterization of antimicrobial peptides from the Caribbean frog, Leptodactylus validus (Anura: Leptodactylidae)

Jay King , Jérôme Leprince (1, 2, 3) , Hubert Vaudry (1) , Laurent Coquet (4) , Thierry Jouenne (4) , J Michael Conlon (5) , J. Michael Conlon

1 Neuroendocrinologie cellulaire et moléculaire
2 DC2N - Différenciation et communication neuronale et neuroendocrine
3 DC2N - Différenciation et communication neuronale et neuroendocrine
4 PBM - Polymères, biopolymères, membranes
5 Faculty of Medicine and Health Science

Peptidomic analysis of norepinephrine-stimulated skin secretions from the Caribbean frog Leptodactylus validus Garman, 1888 led to the identification of three peptides with previously undescribed sequences that were structurally similar to those of antimicrobial peptides isolated from other species of leptodactylid frogs. These paralogs have been termed ocellatin-V1 (GVVDILKGAGKDLLAHALSKLSEKV.NH(2)), ocellatin-V2 (GVLDILKGAGKDLLAHALSKISEKV.NH(2)), and ocellatin-V3 (GVLDILTGAGKDLLAHALSKLSEKV.NH(2)). The very low antimicrobial potency (MIC>200microM) against Escherichia coli and Staphylococcus aureus associated with the peptides is probably a consequence of their lack of amphipathicity and reduced cationicity compared with active members of the ocellatin family from related species.

Domains: Medicinal Chemistry; Cell Behavior [q-bio.CB]; Neurobiology; Pharmacology

Jay King, Jérôme Leprince, Hubert Vaudry, Laurent Coquet, Thierry Jouenne, et al.. Purification and characterization of antimicrobial peptides from the Caribbean frog, Leptodactylus validus (Anura: Leptodactylidae). Peptides, 2008, 29 (8), pp.1287-1292. ⟨10.1016/j.peptides.2008.04.005⟩. ⟨hal-01962774⟩