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Journal articles
Selenoprotein T is a PACAP-regulated gene involved in intracellular Ca 2+ mobilization and neuroendocrine secretion
Selenoprotein T is a PACAP-regulated gene involved in intracellular Ca2+ mobilization and neuroendocrine secretion.
Selenoprotein T is a PACAP-regulated gene involved in intracellular Ca 2+ mobilization and neuroendocrine secretion
Selenoprotein T is a PACAP-regulated gene involved in intracellular Ca2+ mobilization and neuroendocrine secretion.
Luca Grumolato
1, 2
Hafida Ghzili
1, 2
Maite Montero-Hadjadje
2
Stephane Gasman
3
Jean Lesage
4
Yannick Tanguy
2
Ludovic Galas
5
Djida Aït-Ali
1, 2
Jérôme Leprince
1, 2
Nathalie C. Guérineau
6
Abdel Elkahloun
7
Alain Fournier
8
Didier Vieau
4
Hubert Vaudry
1
Youssef Anouar
2, *
*
Corresponding author
Luca Grumolato 1, 2
Author IdHAL : luca-grumolato ORCID : https://orcid.org/0000-0001-8231-3032
Hafida Ghzili 1, 2
Author
Maite Montero-Hadjadje 2
Author IdHAL : maite-montero-hadjadje ORCID : https://orcid.org/0000-0001-8859-893X
Stephane Gasman 3
Author IdHAL : stephane-gasman ORCID : https://orcid.org/0000-0001-8415-1276
Jérôme Leprince 1, 2
Author IdHAL : jerome-leprince ORCID : https://orcid.org/0000-0002-7814-9927
Nathalie C. Guérineau 6
Author IdHAL : nathalie-c-guerineau IdRef : https://www.idref.fr/06998798X ORCID : https://orcid.org/0000-0003-2517-4210
Abdel G. Elkahloun 7
Author
Alain Fournier 8
Author
1
Neuroendocrinologie cellulaire et moléculaire (Universite de Rouen 76821 MONT-SAINT-AIGNAN CEDEX - France)
- UNIROUEN - Université de Rouen Normandie : U413 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U413 (101, rue de Tolbiac, 75013 Paris - France)
2
DC2N - Différenciation et communication neuronale et neuroendocrine (Place Emile Blondel Faculté des Sciences et Techniques 76821 Mont-Saint-Aignan Cedex - France)
- UNIROUEN - Université de Rouen Normandie : U982 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U982 (101, rue de Tolbiac, 75013 Paris - France)
3
NSN - Neurotransmission et sécrétion neuroendocrine (Centre de Neurochimie 5 rue Blaise Pascal 67084 STRASBOURG CEDEX - France)
- CNRS - Centre National de la Recherche Scientifique : UPR2356 (France)
4
EPS - Environnement périnatal et croissance - EA 4489 (Faculté de Médecine - Département Hospitalo-Universitaire de Recherche Expérimentale (DHURE) - 1, Place de Verdun 59045 Lille Cedex - France)
- Université de Lille : EA4489 (Lille - France)
- CHRU Lille - Centre Hospitalier Régional Universitaire [Lille] (2, avenue Oscar Lambret - 59037 Lille Cedex - France)
5
PRIMACEN - Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (Faculté des Sciences et Techniques Place Emile Blondel 76821 Mont-Saint-Aignan Cedex - France)
- UNIROUEN - Université de Rouen Normandie (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- IRIB - Institute for Research and Innovation in Biomedicine (22 Boulevard Gambetta
76183 Rouen Cedex - France)
- UNIROUEN - Université de Rouen Normandie : FED 4220 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- CNRS - Centre National de la Recherche Scientifique (France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : FED 4220 (101, rue de Tolbiac, 75013 Paris - France)
- UNIROUEN - Université de Rouen Normandie : FED 4220 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
6
IGF - Institut de Génomique Fonctionnelle (141, Rue de la Cardonille 34094 Montpellier cedex 5 - France)
- UM - Université de Montpellier (163 rue Auguste Broussonnet - 34090 Montpellier - France)
- UM1 - Université Montpellier 1 (5, boulevard Henri IV - CS 19044 - 34967 Montpellier cedex - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1191 (101, rue de Tolbiac, 75013 Paris - France)
- UM2 - Université Montpellier 2 - Sciences et Techniques (Place Eugène Bataillon - 34095 Montpellier cedex 5 - France)
- CNRS - Centre National de la Recherche Scientifique : UMR5203 (France)
7
Cancer Genetics Branch (Bethesda, MD - United States)
- National Institute of Health (NIH) (France)
- NHGRI - National Human Genome Research Institute (National Institutes of Health Building 31, Room 4B09 31 Center Drive, MSC 2152 9000 Rockville Pike Bethesda, MD 20892-2152 - United States)
8
MONTREAL HEART INSTITUTE - Montreal Heart Institute (Montreal Heart Institute and University of Montreal, Montreal - Canada)
Abstract : Selenoproteins contain the essential trace element selenium, the deficiency of which is associated with cancer or accelerated aging. Although selenoproteins are thought to be instrumental for the effects of selenium, the biological function of many of these proteins remains unknown. Here, we studied the role of selenoprotein T (SelT), a selenocysteine (Sec) -containing protein with no known function, which we have identified as a novel target gene of the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) during PC12 cell differentiation. SelT was found to be ubiquitously expressed throughout embryonic development and in adulthood in rat. Immunocytochemical analysis revealed that SelT is mainly localized to the endoplasmic reticulum through a hydrophobic domain. PACAP and cAMP induced a rapid and long-lasting increase in SelT gene expression in PC12 cells, in a Ca(2+)-dependent manner. These results suggested a possible role of SelT in PACAP signaling during PC12 cell differentiation. Indeed, overexpression of SelT in PC12 cells provoked an increase in the concentration of intracellular Ca(2+) ([Ca(2+)](i)) that was dependent on the Sec residue. Conversely, SelT gene knockdown inhibited the PACAP-induced increase in [Ca(2+)](i) and reduced hormone secretion. These findings demonstrate the implication of a selenoprotein in the regulation of Ca(2+) homeostasis and neuroendocrine secretion in response to a cAMP-stimulating trophic factor.
Document type :
Journal articles
Complete list of metadata
https://hal-normandie-univ.archives-ouvertes.fr/hal-01962747
Contributor : Jérôme Leprince Connect in order to contact the contributor
Submitted on : Thursday, December 20, 2018 - 6:20:43 PM
Last modification on : Friday, October 22, 2021 - 2:48:04 PM
Contributor : Jérôme Leprince Connect in order to contact the contributor
Submitted on : Thursday, December 20, 2018 - 6:20:43 PM
Last modification on : Friday, October 22, 2021 - 2:48:04 PM
Identifiers
- HAL Id : hal-01962747, version 1
- DOI : 10.1096/fj.06-075820
- PUBMED : 18198219
Collections
CNRS | IGF | BS | UNIV-MONTPELLIER | UNIROUEN | UNIV-LILLE | DC2N | COMUE-NORMANDIE
Citation
Luca Grumolato, Hafida Ghzili, Maite Montero-Hadjadje, Stephane Gasman, Jean Lesage, et al.. Selenoprotein T is a PACAP-regulated gene involved in intracellular Ca 2+ mobilization and neuroendocrine secretion. FASEB Journal, Federation of American Society of Experimental Biology, 2008, 22 (6), pp.1756-1768. ⟨10.1096/fj.06-075820⟩. ⟨hal-01962747⟩
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