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Isolation, Primary Structure, and Effects onα -Melanocyte-Stimulating Hormone Release of Frog Neurotensin 1

Abstract : Neurotensin (NT) was isolated in pure form from the small intestine of the European green frog, Rana ridibunda, and its primary structure was established as pGlu-Ala-His-Ile-Ser-Lys-Ala-Arg-Arg-Pro-Tyr-Ile-Leu. This sequence contains five amino acid substitutions (Leu2-->Ala, Tyr3-->His, Glu4-->Ile, Asn5-->Ser, and Pro7-->Ala) compared with human NT. A peptide with identical chromatographic properties was identified in an extract of frog brain. Synthetic frog NT produced a concentration-dependent increase in alphaMSH release from perifused frog pars intermedia cells, with an ED50 of 5 x 10(-9) M. A maximum response (276.3 +/- 45.5% above basal release) was produced by a 10(-8) M concentration. Repeated administration of NT to melanotrope cells revealed the occurrence of a rapid and pronounced desensitization mechanism. The data are consistent with a possible role for the peptide as a hypophysiotropic factor in amphibians.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01962694
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Submitted on : Thursday, December 20, 2018 - 5:46:50 PM
Last modification on : Thursday, February 7, 2019 - 5:46:20 PM

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Laurence Desrues, Marie-Christine Tonon, Jérôme Leprince, Hubert Vaudry, J. Michael Conlon. Isolation, Primary Structure, and Effects onα -Melanocyte-Stimulating Hormone Release of Frog Neurotensin 1. Endocrinology, Endocrine Society, 1998, 139 (10), pp.4140-4146. ⟨10.1210/endo.139.10.6255⟩. ⟨hal-01962694⟩

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