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Glutamine Regulates the Human Epithelial Intestinal HCT-8 Cell Proteome under Apoptotic Conditions

Abstract : Glutamine plays a key role in the metabolism of rapidly dividing cells, including enterocytes and lymphocytes, which may contribute to its beneficial clinical effects. Gut mucosal homeostasis is achieved through a balance between cell proliferation and apoptosis. In T cells, glutamine up-regulates antiapoptotic proteins and down-regulates proapoptotic proteins. In gut mucosa, glutamine prevents apoptosis in rat epithelial cell lines, whereas glutamine starvation induces apoptosis through caspase activation. Finally glutamine specifically prevents tumor necrosis factor-alpha-related apoptosis in the human intestinal cell line HT-29. Comparative functional proteomics enables the characterization of each differentially expressed protein in intestinal cells in response to modifications of nutritional environment. The influence of glutamine on intestinal proteome expression in apoptotic conditions has not been studied and evaluated. This comparative proteomics study was performed in the human epithelial intestinal cell line HCT-8 under experimental apoptotic conditions to investigate the influence of glutamine on protein expression during apoptosis. The pharmaconutritional effects of glutamine were determined under 2 mm (physiological concentration) and 10 mm (pharmaconutritional concentration) conditions. About 1,800 protein spots were revealed in both conditions. Comparative assessments indicated that 28 proteins were differentially expressed significantly (i.e. at least 2-fold modulated and Student's t test with p
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01962690
Contributor : Jérôme Leprince <>
Submitted on : Thursday, December 20, 2018 - 5:45:20 PM
Last modification on : Tuesday, April 7, 2020 - 5:08:05 PM

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Nicolas Deniel, Rachel Marion-Letellier, Roland Charlionet, François Tron, Jérôme Leprince, et al.. Glutamine Regulates the Human Epithelial Intestinal HCT-8 Cell Proteome under Apoptotic Conditions. Molecular and Cellular Proteomics, American Society for Biochemistry and Molecular Biology, 2007, 6 (10), pp.1671-1679. ⟨10.1074/mcp.M600428-MCP200⟩. ⟨hal-01962690⟩

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