Skip to Main content Skip to Navigation
Journal articles

Evidence from peptidomic analysis of skin secretions that the red-legged frogs, Rana aurora draytonii and Rana aurora aurora, are distinct species

Abstract : The northern red-legged frog Rana aurora aurora and the California red-legged frog Rana aurora draytonii are traditionally classified together in the same species group. Ten peptides with antimicrobial activity were isolated from norepinephrine-stimulated skin secretions of R. aurora draytonii and purified to near homogeneity. The peptides were identified as belonging to the ranatuerin-2 family (two peptides), brevinin-1 family (four peptides), temporin family (three peptides), and a novel peptide, RV-23 (RIGVLLARLPKLFSLFKLMGKKV) that has limited structural similarity to the bee venom peptide, melittin. This distribution of peptides contrasts with that found previously in skin secretions from R. aurora aurora collected under the same conditions and at the same time of year (one ranatuerin-2 peptide, two brevinin-1 peptides, and one temporin peptide). The variation in amino acid sequences between corresponding R. aurora draytonii and R. aurora aurora peptides is comparable with the variation in sequences of orthologs from other members of the Amerana group of New World ranid frogs (Rana boylii, Rana muscosa, and Rana luteiventris). It is proposed, therefore, that the red-legged frogs should be regarded as separate species (R. aurora and R. draytonii) within the Amerana group rather than conspecific subspecies. The data emphasize that amino acid sequences of antimicrobial peptides in skin secretions may be used to infer taxonomic and phylogenetic relationships between species of ranid frogs.
Complete list of metadatas

https://hal-normandie-univ.archives-ouvertes.fr/hal-01960885
Contributor : Jérôme Leprince <>
Submitted on : Wednesday, December 19, 2018 - 3:59:47 PM
Last modification on : Tuesday, April 7, 2020 - 3:36:04 PM

Identifiers

Citation

J Michael Conlon, Nadia Al-Ghafari, Laurent Coquet, Jérôme Leprince, Thierry Jouenne, et al.. Evidence from peptidomic analysis of skin secretions that the red-legged frogs, Rana aurora draytonii and Rana aurora aurora, are distinct species. Peptides, Elsevier, 2006, 27 (6), pp.1305-1312. ⟨10.1016/j.peptides.2005.10.018⟩. ⟨hal-01960885⟩

Share

Metrics

Record views

45