A proenkephalin A-derived peptide analogous to bovine adrenal peptide E from frog brain: purification, synthesis, and behavioral effects.

J Conlon , C Chartrel , Jérôme Leprince (1, 2, 3) , C Suaudeau , J. Costentin (4) , H. Vaudry (1)

1 Neuroendocrinologie cellulaire et moléculaire
2 DC2N - Différenciation et communication neuronale et neuroendocrine
3 DC2N - Différenciation et communication neuronale et neuroendocrine
4 Neuropsycho-pharmacologie expérimentale

A peptide derived from the posttranslational processing of proenkephalin A was isolated from an extract of the brain of the European green frog Rana ridibunda and its primary structure established as: Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly-Arg10- Pro-Glu-Trp-Trp-Gln-Asp-Tyr-Gln-Lys-Arg20-Tyr-Gly-Gly-Phe-Met. The structure was confirmed by chemical synthesis. The peptide represents an amphibian equivalent of bovine adrenal peptide E [preproenkephalin A (206-230)-peptide] but the sequence contains two amino acid substitutions (Met15-->Gln and Leu25-->Met) compared with the mammalian peptide. The data support previous hypotheses that the Leu-enkephalin sequence is not present in preproenkephalin A of amphibians. Intracerebroventricular injections of frog peptide E (10 and 100 ng) in mice had no significant effect on horizontal locomotor activity. The peptide, in doses up to 1 microgram, had no effect on latency of escape jumping in the hot plate test and the peptide (100 ng) did not modify responses (paw licking, rearing, and escape jumping) in morphine-treated mice.

Domaines: Chimie thérapeutique; Interactions cellulaires [q-bio.CB]; Neurobiologie; Pharmacologie

J Conlon, C Chartrel, Jérôme Leprince, C Suaudeau, J. Costentin, et al.. A proenkephalin A-derived peptide analogous to bovine adrenal peptide E from frog brain: purification, synthesis, and behavioral effects.. Peptides, 1996, 17 (8), pp.1291-6. ⟨hal-01960880⟩