In vivo action of a new octadecaneuropeptide (ODN) antagonist on gonadotropin-releasing hormone gene expression in the male rat brain

M Compère , S. Li (1) , Jérôme Leprince (2, 3, 4) , M.C Tonon , H. Vaudry (2) , G. Pelletier (5) , V Compère , V Vaudry

1 ING - IRCELYON-Ingéniérie, du matériau au réacteur
2 Neuroendocrinologie cellulaire et moléculaire
3 DC2N - Différenciation et communication neuronale et neuroendocrine
4 DC2N - Différenciation et communication neuronale et neuroendocrine
5 Chercheur indépendant

It has been reported that several of the effects induced by octadecaneuropeptide (ODN) could be mediated by an activation of a metabotropic receptor. In order to investigate the role and mechanism of action of ODN in gonadotropin-releasing hormone (GnRH) neuron regulation, we studied the effects of the acute i.c.v. administration of ODN and of a new ODN antagonist to metabotropic receptor, cyclo(1-8)[Dleu(5)]OP, on GnRH mRNA expression as evaluated by in situ hybridization in castrated male rats. The administration of ODN produced a decrease in the hybridization signal while the administration of cyclo(1-8)[Dleu(5)]OP alone produced an 18% increase. When administrated concomitantly with ODN, the antagonist both inhibited the depressing effect of ODN and induced a 22% increase over the values detected in ODN-treated rats. The data suggest that the effect of ODN on GnRH mRNA expression might be mediated by interaction with metabotropic receptors.

Domains: Chemical Sciences; Medicinal Chemistry; Life Sciences [q-bio]; Cellular Biology; Cell Behavior [q-bio.CB]; Life Sciences [q-bio]; Neurons and Cognition [q-bio.NC]; Neurobiology; Life Sciences [q-bio]; Pharmaceutical sciences; Pharmacology

M Compère, S. Li, Jérôme Leprince, M.C Tonon, H. Vaudry, et al.. In vivo action of a new octadecaneuropeptide (ODN) antagonist on gonadotropin-releasing hormone gene expression in the male rat brain. Neuroscience, 2004, 125 (2), pp.411-415. ⟨10.1016/j.neuroscience.2004.02.016⟩. ⟨hal-01960863⟩