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Journal articles
Purification, structural characterization, and myotropic activity of a peptide related to des-Arg9-bradykinin from an elasmobranch fish, the little skate, Leucoraja erinacea
Abstract : A bradykinin (BK)-related peptide was isolated from heat-denaturated plasma from an elasmobranch fish, the little skate, Leucoraja erinacea after incubation with porcine pancreatic kallikrein. The primary structure of the peptide (H-Gly-Ile-Thr-Ser-Trp-Leu-Pro-Phe-OH; skate BK) shows limited structural similarity to the mammalian B1 receptor agonist, des-Arg(9)-BK. The myotropic activities of synthetic skate BK, and the analog skate [Arg(9)]BK, were examined in isolated skate vascular and intestinal smooth muscle preparations. Skate BK produced a concentration-dependent constriction of the mesenteric artery (EC(50)=4.37x10(-8)M; maximum response=103.4+/-10.23% of the response to 60mM KCl) but the response to skate [Arg(9)]BK was appreciably weaker (response to 10(-6)M=73.0+/-23.4% of the response to 60mM KCl). Neither the first branchial gill arch nor the ventral aorta responded to either purified peptide. Skate BK also produced a concentration-dependent constriction of intestinal smooth muscle preparations (EC(50)=2.74x10(-7)M; maximum response 31.0+/-12.2% of the response to 10(-5)M acetylcholine). Skate [Arg(9)]BK was without effect on the intestinal preparation. The data provide evidence for the existence of the kallikrein-kinin system in a phylogenetically ancient vertebrate group and the greater potency of skate BK compared with the analog skate [Arg(9)]BK suggests that the receptor mediating vascular responses resembles the mammalian B1 receptor more closely than the B2 receptor.
https://hal-normandie-univ.archives-ouvertes.fr/hal-01960630
Contributor : Jérôme Leprince <>
Submitted on : Wednesday, December 19, 2018 - 2:58:03 PM
Last modification on : Tuesday, April 7, 2020 - 3:36:04 PM
Contributor : Jérôme Leprince <>
Submitted on : Wednesday, December 19, 2018 - 2:58:03 PM
Last modification on : Tuesday, April 7, 2020 - 3:36:04 PM
