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Peptides with potent cytolytic activity from the skin secretions of the North American leopard frogs, Lithobates blairi and Lithobates yavapaiensis

C Condamine Karine Courchay Jean Rego Catherine Mayer 1 Daniel Davoust 2 Jean-Claude Do Rego 3 C Chartrel C Suaudeau Jean Costentin 4 Agnes Sonnevend Nadia Al-Ghafari Carlos Davidson Pascal Cosette 5 Per Nielsen 6 Paul Moler Catherine Bevier Blake Hossack Nadia Al-Ghaferi Bency Abraham Douglas Woodhams Haider Raza 7 Louise Rollins-Smith Shawichi Iwamuro Jolanta Kolodziejek Norbert Nowotny 8 Gavin Power Hu Jiansheng Hu Vaudry Yasser Abdel-Wahab Peter Flatt Eric Condamine 9 Yasser H.A. Abdel-Wahab J Michael Conlon 10 Eman Ahmed Laurent Coquet 11 Thierry Jouenne 11 Jérôme Leprince 12, 13, 14 Hubert Vaudry 12 Jay King J. Michael Conlon
1 Commission for Geodesy and Glaciology
2 ECOBS - Equipe de Chimie Organique et Biologie Structurale
INSA Rouen Normandie - Institut national des sciences appliquées Rouen Normandie
3 SCAC - Service Commun d'Analyse Comportementale
4 Neuropsycho-pharmacologie expérimentale
5 PBS - Polymères Biopolymères Surfaces
6 KU - University of Copenhagen = Københavns Universitet
7 University of Ulster
8 Zoonoses and Emerging Infections Group
9 GIN - Grenoble Institut des Neurosciences
10 Faculty of Medicine and Health Science
11 PBM - Polymères, biopolymères, membranes
12 Neuroendocrinologie cellulaire et moléculaire
13 DC2N - Différenciation et communication neuronale et neuroendocrine
14 DC2N - Différenciation et communication neuronale et neuroendocrine
Abstract : Six structurally similar and strongly cationic peptides belonging to the brevinin-1 family were isolated from skin secretions of the plains leopard frog Lithobates blairi and the lowland leopard frog Lithobates yavapaiensis on the basis of their antimicrobial activities. Brevinin-1BLc (FLPIIAGIAAKFLPKIFCTISKKC) from L. blairi represented the most potent peptide (MIC=25microM Escherichia coli, MIC=1.5microM Staphylococcus aureus, MIC=3microM Candida albicans, LC(50)=9microM human erythrocytes and LC(50)=6microM HepG2 human hepatoma-derived cells). The appreciably lower antimicrobial potencies of brevinin-1Ya and -1Yc from L. yavapaiensis correlate with the decreases in cationicity produced by the amino acid substitutions Lys(11)-->Asn (brevinin-1Ya) and Pro(14)-->Glu (brevinin-1Yc). In addition, a peptide isolated from the skin secretions of L. yavapaiensis belonging to the ranatuerin-2 family (ranatuerin-2Ya; GLMDTIKGVAKTVAASWLDKLKCKIT GC) inhibited the growth of E. coli (MIC=50microM) and S. aureus (MIC=50microM). In contrast to brevinin-1BLc, ranatuerin-2Ya showed appreciably greater cytolytic activity against HepG2 cells (LC(50)=20microM) than against erythrocytes (LC(50)>100microM).
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01960586
Contributor : Jérôme Leprince <>
Submitted on : Wednesday, December 19, 2018 - 2:44:12 PM
Last modification on : Thursday, July 2, 2020 - 3:29:27 AM

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COMUE-NORMANDIE | CNRS | UGA | INSA-ROUEN | UNIROUEN | DC2N | INSA-GROUPE | PBS | ENSICAEN | UNILEHAVRE | UNICAEN

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C Condamine, Karine Courchay, Jean Rego, Catherine Mayer, Daniel Davoust, et al.. Peptides with potent cytolytic activity from the skin secretions of the North American leopard frogs, Lithobates blairi and Lithobates yavapaiensis. Toxicon, Elsevier, 2009, 53 (7-8), pp.699-705. ⟨10.1016/j.toxicon.2009.02.018⟩. ⟨hal-01960586⟩

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