Skip to Main content Skip to Navigation
Journal articles

A new study of the bacterial lipidome: HPTLC-MALDI-TOF imaging enlightening the presence of phosphatidylcholine in airborne Pseudomonas fluorescens MFAF76a

Abstract : Lipids are major functional components of bacterial cells that play fundamental roles in bacterial metabolism and the barrier function between cells and the environment. In an effort to investigate the bacterial lipidome, we adopted a protocol using MALDI-TOF MS imaging coupled to HPTLC to screen a large number of phospholipid classes in a short span of time. With this method, phospholipids of airborne Pseudomonas fluorescens MFAF76a were visualized and identified in sample extracts (measurement accuracy below 0.1 Da, phospholipid identification by means of four characteristic fragment peaks). Via this technique, the P. fluorescens lipidome was shown to comprise three major lipid classes: phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The protocol described herein is simple, rapid and effective for screening of bacterial phospholipid classes. The remarkable presence of a eukaryotic phospholipid, phosphatidylcholine, was observed in P. fluorescens MFAF76a. This lipid is known to play a role in bacteria–host interactions and had not been known to be found in P. fluorescens cells.
Complete list of metadatas

https://hal-normandie-univ.archives-ouvertes.fr/hal-01939158
Contributor : Cécile Duclairoir Poc <>
Submitted on : Thursday, November 29, 2018 - 11:26:43 AM
Last modification on : Thursday, July 30, 2020 - 3:09:51 AM

Identifiers

Citation

Tatiana Kondakova, Nadine Merlet-Machour, Manuel Chapelle, David Préterre, Frédéric Dionnet, et al.. A new study of the bacterial lipidome: HPTLC-MALDI-TOF imaging enlightening the presence of phosphatidylcholine in airborne Pseudomonas fluorescens MFAF76a. Research in Microbiology, Elsevier, 2015, 166 (1), pp.1 - 8. ⟨10.1016/j.resmic.2014.11.003⟩. ⟨hal-01939158⟩

Share

Metrics

Record views

375