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N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures

Abstract : Quorum sensing (QS) systems, which depend on N-acylhomoserine lactone (AHL) signal molecules, mediate the production of virulence factors in many pathogenic microorganisms. One hundred and forty-six bacterial strains, isolated from a bivalve hatchery, were screened for their capacity to degrade five synthetic AHLs [N-butyryl-DL-homoserine lactone (C4-HSL), N-hexanoyl-DL-homoserine lactone (C6-HSL), N-octanoyl-DL-homoserine lactone (C8-HSL), N-decanoyl-DL-homoserine lactone (C10-HSL) and N-dodecanoyl-DL-homoserine lactone (C12-HSL)] using well diffusion agar-plate assays with three biosensors, Chromobacterium violaceum CV026, C. violaceum VIR07 and Agrobacterium tumefaciens NTL4 (pZLR4). The results of these assays led to our choosing four strains (PP2-67, PP2-459, PP2-644 and PP2-663) that were able to degrade all five synthetic AHLs, thus showing a wide spectrum of quorum quenching (QQ) activity. We subsequently confirmed and measured the QQ activity of the four strains by high-performance liquid chromatography plus mass-spectrometry analysis (HPLC-MS). One of the strains which showed the highest AHL-degrading activity, PP2-459, identified as being a member of the genus Thalassomonas was chosen for further study. Finally, using thin-layer chromatography (TLC), we went on to confirm this strain's capacity to degrade the AHLs produced by other non-pathogenic and pathogenic bacteria not taxonomically related.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01938098
Contributor : Ali Tahrioui <>
Submitted on : Wednesday, November 28, 2018 - 2:35:49 PM
Last modification on : Sunday, November 24, 2019 - 4:14:02 PM

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Marta Torres, Manuel Romero, Susana Prado, Javier Dubert, Ali Tahrioui, et al.. N-acylhomoserine lactone-degrading bacteria isolated from hatchery bivalve larval cultures. Microbiological Research, Elsevier, 2013, 168 (9), pp.547 - 554. ⟨10.1016/j.micres.2013.04.011⟩. ⟨hal-01938098⟩

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