Skip to Main content Skip to Navigation
Journal articles

Probe, Sample, and Instrument (PSI): The Hat-Trick for Fluorescence Live Cell Imaging

Abstract : Cell Imaging Platforms (CIPs) are research infrastructures offering support to a number of scientific projects including the choice of adapted fluorescent probes for live cell imaging. What to detect in what type of sample and for how long is a major issue with fluorescent probes and, for this, the “hat-trick” “Probe–Sample–Instrument” (PSI) has to be considered. We propose here to deal with key points usually discussed in CIPs including the properties of fluorescent organic probes, the modality of cell labeling, and the best equipment to obtain appropriate spectral, spatial, and temporal resolution. New strategies in organic synthesis and click chemistry for accessing probes with enhanced photophysical characteristics and targeting abilities will also be addressed. Finally, methods for image processing will be described to optimize exploitation of fluorescence signals.
Complete list of metadata

Cited literature [277 references]  Display  Hide  Download
Contributor : Arnaud Lehner Connect in order to contact the contributor
Submitted on : Friday, December 6, 2019 - 2:10:32 PM
Last modification on : Saturday, June 25, 2022 - 9:55:03 AM


Publisher files allowed on an open archive


Distributed under a Creative Commons Attribution 4.0 International License



Ludovic Galas, Thibault Gallavardin, Magalie Benard, Arnaud Lehner, Damien Schapman, et al.. Probe, Sample, and Instrument (PSI): The Hat-Trick for Fluorescence Live Cell Imaging. Chemosensors, MDPI, 2018, 6 (3), pp.40. ⟨10.3390/chemosensors6030040⟩. ⟨hal-01874039⟩



Record views


Files downloads