Probe, Sample, and Instrument (PSI): The Hat-Trick for Fluorescence Live Cell Imaging

Ludovic Galas (1) , Thibault Gallavardin (2) , Magalie Benard (1) , Arnaud Lehner (3) , Damien Schapman (1) , Alexis Lebon (1) , Hitoshi Komuro (4) , Patrice Lerouge (3) , Stéphane Leleu (2) , Xavier Franck (2)

1 PRIMACEN - Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie
2 COBRA - Chimie Organique et Bioorganique : Réactivité et Analyse
3 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
4 Yale University [New Haven]

Cell Imaging Platforms (CIPs) are research infrastructures offering support to a number of scientific projects including the choice of adapted fluorescent probes for live cell imaging. What to detect in what type of sample and for how long is a major issue with fluorescent probes and, for this, the “hat-trick” “Probe–Sample–Instrument” (PSI) has to be considered. We propose here to deal with key points usually discussed in CIPs including the properties of fluorescent organic probes, the modality of cell labeling, and the best equipment to obtain appropriate spectral, spatial, and temporal resolution. New strategies in organic synthesis and click chemistry for accessing probes with enhanced photophysical characteristics and targeting abilities will also be addressed. Finally, methods for image processing will be described to optimize exploitation of fluorescence signals.

Keywords: fluorescent probes; advanced microscopy; instrumental performances; small fluorescent organic molecules; fluorescent proteins; cell labeling; live cell imaging; photophysical properties; organic synthesis; click chemistry; image processing

Domains: Chemical Sciences; Polymers; Life Sciences [q-bio]; Vegetal Biology; Plant breeding; Life Sciences [q-bio]; Cellular Biology; Cell Behavior [q-bio.CB]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Biochemistry [q-bio.BM]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular biology; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular Networks [q-bio.MN]; Life Sciences [q-bio]; Vegetal Biology; Phytopathology and phytopharmacy; Life Sciences [q-bio]; Biotechnology; Life Sciences [q-bio]; Development Biology; Gametogenesis; Life Sciences [q-bio]; Cellular Biology; Subcellular Processes [q-bio.SC]

Ludovic Galas, Thibault Gallavardin, Magalie Benard, Arnaud Lehner, Damien Schapman, et al.. Probe, Sample, and Instrument (PSI): The Hat-Trick for Fluorescence Live Cell Imaging. Chemosensors, 2018, 6 (3), pp.40. ⟨10.3390/chemosensors6030040⟩. ⟨hal-01874039⟩