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Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity

Abstract : Response surface methodology using a Box-Behnken design was employed to optimize extraction temperature, extraction time and ratio of water to material to obtain a maximum polysaccharide yield with high uronic acid content and antioxidant property from edible Zizyphus lotus fruit. The optimal conditions were: extraction time of 3 h 15 min, extraction temperature of 91.2 °C and water to solid ratio of 39 mL/g. Under these conditions, the experimental extraction yield, uronic acid content and 2,2-diphenyl-1-picrylhydrazyl scavenging ability (IC50) were 18.88%, 41.89 and 0.518 mg/mL, respectively. Chemical analysis revealed that the extract was composed of 97.92% carbohydrate of which 41.89% is uronic acid. The extracted polysaccharides, with an average molecular weight of 2720 kDa, are composed of arabinose, rhamnose, glucose, fructose, galactose and xylose. Moreover, the polysaccharides exhibited a significant reducing power and anti-lipid peroxidation activities.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01869040
Contributor : Didier Le Cerf <>
Submitted on : Thursday, September 6, 2018 - 11:02:07 AM
Last modification on : Friday, October 23, 2020 - 7:02:01 PM

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Khaoula Mkadmini Hammi, Majdi Hammami, Christophe Rihouey, Didier Le Cerf, Riadh Ksouri, et al.. Optimization extraction of polysaccharide from Tunisian Zizyphus lotus fruit by response surface methodology: Composition and antioxidant activity. Food Chemistry, Elsevier, 2016, 212, pp.476 - 484. ⟨10.1016/j.foodchem.2016.06.004⟩. ⟨hal-01869040⟩

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