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Physico-chemical characterization and pharmacological activities of sulfated polysaccharide from sea urchin, Paracentrotus lividus

Abstract : Sulfated polysaccharide (SP) from the eggs of sea urchin Paracentrotus lividus, extracted by papain digestion, was characterized by size exclusion chromatography coupling on-line with light scattering and viscosity detectors (SEC/MALS/VD/DRI), gas chromatography coupled to mass spectrometer (GC–MS), and Fourier transform infrared spectroscopy (FTIR) analysis. The native molecular mass of the extracted polysaccharide is high (≥22 000 KDa) and it is composed mainly of arabinose, accompanied by other monosaccharides (mostly galactose, glucose and fucose), significant amounts of uronic acids (18.4%) and relatively high proportions of sulfate (22.4%). The pharmacological evaluation of SP showed a significant in vivo anti-inflammatory activity (p < 0.001), 3 h after injection, the edema inhibition was 75.8% at the dose of 100 mg/Kg; a significant peripheral analgesic activity (p < 0.001), with 64.9% of writhing inhibition, and a significant increase in the hot plate reaction time in mice indicating central analgesic activity. In addition, an interesting gastroprotective effect was observed with this polysaccharide; the gastric ulcer inhibition was 69.7%, at the dose of 100 mg/Kg.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01869032
Contributor : Didier Le Cerf <>
Submitted on : Thursday, September 6, 2018 - 10:59:33 AM
Last modification on : Thursday, October 22, 2020 - 3:50:04 PM

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Yosra Ben Salem, Safa Amri, Khaoula Mkadmini Hammi, Amal Abdelhamid, Didier Le Cerf, et al.. Physico-chemical characterization and pharmacological activities of sulfated polysaccharide from sea urchin, Paracentrotus lividus. International Journal of Biological Macromolecules, Elsevier, 2017, 97, pp.8 - 15. ⟨10.1016/j.ijbiomac.2017.01.007⟩. ⟨hal-01869032⟩

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