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Analysis of a complex polysaccharide (gum arabic) by multi-angle laser light scattering coupled on-line to size exclusion chromatography and flow field flow fractionation

Abstract : The heterogeneous polysaccharide gum arabic has been characterized using size exclusion chromatography (SEC) and flow field flow fractionation (F4) coupled on-line to multi-angle laser light scattering (MALLS). Two distinct populations have been shown. About 80% of the material consist of highly branched arabinogalactan (AG) units. The rest is mainly composed of heterogeneous arabinogalactan–protein complex (AGP) of high molecular weight. The F4/MALLS method is shown to allow the complete analysis of gum arabic whereas SEC gave only partial information. From light-scattering-based radii of gyration (Rg) and F4-based hydrodynamic radii (Rh) we found that the ratio Rg/Rh is near 0.8 for AGP. This value is reasonably consistent with a globular conformation and gives further evidence in favour of the “wattle blossom model” proposed by Connolly et al.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01866923
Contributor : Luc Picton <>
Submitted on : Monday, September 3, 2018 - 4:59:32 PM
Last modification on : Wednesday, December 4, 2019 - 11:18:23 AM

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L. Picton, I. Bataille, G. Muller. Analysis of a complex polysaccharide (gum arabic) by multi-angle laser light scattering coupled on-line to size exclusion chromatography and flow field flow fractionation. Carbohydrate Polymers, Elsevier, 2000, 42 (1), pp.23 - 31. ⟨10.1016/s0144-8617(99)00139-3⟩. ⟨hal-01866923⟩

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