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Aggregation of amphiphilic pullulan derivatives evidenced by on-line flow field flow fractionation/multi-angle laser light scattering

Abstract : Size-exclusion chromatography (SEC) is a useful steric separation technique for the analysis of water-soluble polysaccharides in aqueous solution. However, in the case of amphiphilic derivatives, the usefulness is limited because of interactions between hydrophobic segments and the stationary phase. Alkyl-bearing pullulans differing from the extent and the length of alkyl groups were characterized using flow-field flow fractionation with on-line coupling multi-angle laser light scattering (F4/MALLS). Comparison of SEC and F4 is presented and the interest of F4 in the field of amphiphilic derivatives is demonstrated.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-01866917
Contributor : Luc Picton <>
Submitted on : Monday, September 3, 2018 - 4:55:36 PM
Last modification on : Wednesday, December 4, 2019 - 11:18:19 AM

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Christian Duval, D. Le Cerf, L. Picton, G. Muller. Aggregation of amphiphilic pullulan derivatives evidenced by on-line flow field flow fractionation/multi-angle laser light scattering. Journal of Chromatography B Biomedical Sciences and Applications, Elsevier, 2001, 753 (1), pp.115 - 122. ⟨10.1016/s0378-4347(00)00517-x⟩. ⟨hal-01866917⟩

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