Curdlan microspheres. Synthesis, characterization and interaction with proteins (enzymes, vaccines)

Georgeta Mocanu (1) , Doina Mihai (1) , Misu Moscovici (2) , Luc Picton (3) , Didier Lecerf (3)

1 “Petru Poni” Institute of Macromolecular Chemistry
2 ICCF - National Institute for Chemical-Pharmaceutical Research and Development
3 PBS - Polymères Biopolymères Surfaces

Microparticles of curdlan, synthesized through crosslinking with epichlorohydrin in organic suspension media, were chemically modified with the aim of introducing strongly and/or weakly acidic anionic and palmitoyl hydrophobic groups. Microparticles of both curdlan and curdlan derivatives were physico-chemically characterized. Study of the interaction with enzymes, such as lysozyme, and vaccines, such as tetanus anatoxin, showed a co-operative protein retention effect, induced by electrostatic and hydrophobic forces. The results of the in vitro release studies on support–protein complexes recommend them as potential controlled release systems.

Mots clés: Curdlan; Microparticles; Lysozyme; Tetanus anatoxin; Controlled release systems

Domaines: Chimie; Polymères

Georgeta Mocanu, Doina Mihai, Misu Moscovici, Luc Picton, Didier Lecerf. Curdlan microspheres. Synthesis, characterization and interaction with proteins (enzymes, vaccines). International Journal of Biological Macromolecules, 2009, 44 (3), pp.215 - 221. ⟨10.1016/j.ijbiomac.2008.12.005⟩. ⟨hal-01866796⟩