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Journal articles
Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction
Abstract : Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.
https://hal-normandie-univ.archives-ouvertes.fr/hal-01866736
Contributor : Luc Picton Connect in order to contact the contributor
Submitted on : Thursday, May 27, 2021 - 4:20:21 PM
Last modification on : Tuesday, October 19, 2021 - 11:34:46 PM
Long-term archiving on: : Saturday, August 28, 2021 - 7:53:28 PM
Contributor : Luc Picton Connect in order to contact the contributor
Submitted on : Thursday, May 27, 2021 - 4:20:21 PM
Last modification on : Tuesday, October 19, 2021 - 11:34:46 PM
Long-term archiving on: : Saturday, August 28, 2021 - 7:53:28 PM
File
marinedrugs-12-04851.pdf
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Distributed under a Creative Commons Attribution 4.0 International License
