Skip to Main content Skip to Navigation
Journal articles

Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction

Abstract : Fucoidans constitute a large family of sulfated polysaccharides with several biochemical properties. A commercial fucoidan from brown algae, containing low molecular weight polysaccharidic species constituted of l-fucose, uronic acids and sulfate groups, was simply treated here with calcium acetate solution. This treatment led to a purified fraction with a yield of 45%. The physicochemical characterizations of the purified fucoidan using colorimetric assay, MALLS, dRI, FT-IR, NMR, exhibited molecular weight distributions and chemical profiles similar for both fucoidans whereas the sulfate and l-fucose contents increased by 16% and 71%, respectively. The biodistribution study in rat of both compounds labeled with 99mTc evidenced a predominant renal elimination of the purified fucoidan, but the crude fucoidan was mainly retained in liver and spleen. In rat myocardial ischemia-reperfusion, we then demonstrated the better efficiency of the purified fucoidan. This purified sulfated polysaccharide appears promising for the development of molecular imaging in acute coronary syndrome.
Document type :
Journal articles
Complete list of metadata
Contributor : Luc Picton Connect in order to contact the contributor
Submitted on : Thursday, May 27, 2021 - 4:20:21 PM
Last modification on : Saturday, June 25, 2022 - 9:56:43 AM
Long-term archiving on: : Saturday, August 28, 2021 - 7:53:28 PM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License



Pierre Saboural, Frederic Chaubet, François Rouzet, Faisal Al-Shoukr, Rana Azzouna, et al.. Purification of a Low Molecular Weight Fucoidan for SPECT Molecular Imaging of Myocardial Infarction. Marine drugs, MDPI, 2014, 12 (9), pp.4851-4867. ⟨10.3390/md12094851⟩. ⟨hal-01866736⟩



Record views


Files downloads