Intrinsic spatial shift of local focus metric curves in digital inline holography for accurate 3D morphology measurement of irregular micro-objects

Yingchun Wu (1, 2, 3) , Xuecheng Wu (1) , Denis Lebrun (2) , Marc Brunel (2) , Sébastien Coëtmellec (2) , Olivier Lesouhaitier (4) , Jiaji Chen (3) , Gérard Gréhan (2)

1 Zhejiang University
2 CORIA - Complexe de recherche interprofessionnel en aérothermochimie
3 TUM - Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne]
4 LMSM - Laboratoire de Microbiologie Signaux et Microenvironnement

A theoretical model of digital inline holography system reveals that the local focus metric curves (FMCs) of different parts of an irregular micro-object present spatial shift in the depth direction which is resulted from the depth shift. Thus, the 3D morphology of an irregular micro-object can be accurately measured using the cross correlation of the local FMCs. This method retrieves the 3D depth information directly, avoiding the uncertainty inherited from the depth position determination. Typical 3D morphology measurements, including the 3D boundary lines of tilted carbon fibers and irregular coal particles, and the 3D swimming gesture of a live Caenorhabdities elegans, are presented.

Domains: Engineering Sciences [physics]; Optics / Photonic

Yingchun Wu, Xuecheng Wu, Denis Lebrun, Marc Brunel, Sébastien Coëtmellec, et al.. Intrinsic spatial shift of local focus metric curves in digital inline holography for accurate 3D morphology measurement of irregular micro-objects. Applied Physics Letters, 2016, 109 (12), ⟨10.1063/1.4963131⟩. ⟨hal-01861350⟩