Golgi-Mediated Synthesis and Secretion of Matrix Polysaccharides of the Primary Cell Wall of Higher Plants

Azeddine Driouich (1) , Marie-Laure Follet-Gueye (1) , Sophie Bernard (1) , Sumaira Kousar (2) , Laurence Chevalier (3) , Maïté Vicré-Gibouin (1) , Olivier Lerouxel (2)

1 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2 CERMAV - Centre de Recherches sur les Macromolécules Végétales
3 GPM - Groupe de physique des matériaux

The Golgi apparatus of eukaryotic cells is known for its central role in the processing, sorting , and transport of proteins to intra-and extra-cellular compartments. In plants, it has the additional task of assembling and exporting the non-cellulosic polysaccharides of the cell wall matrix including pectin and hemicelluloses, which are important for plant development and protection. In this review, we focus on the biosynthesis of complex polysaccharides of the primary cell wall of eudicotyledonous plants. We present and discuss the compartmen-tal organization of the Golgi stacks with regards to complex polysaccharide assembly and secretion using immuno-electron microscopy and specific antibodies recognizing various sugar epitopes. We also discuss the significance of the recently identified Golgi-localized glycosyltransferases responsible for the biosynthesis of xyloglucan (XyG) and pectin.

Keywords: plants; polysaccharides; Golgi; glycosyltransferases; immunocytochemistry; cell wall

Domains: Life Sciences [q-bio]; Biochemistry, Molecular Biology; Biochemistry [q-bio.BM]; Life Sciences [q-bio]; Vegetal Biology; Botanics; Chemical Sciences; Polymers

Azeddine Driouich, Marie-Laure Follet-Gueye, Sophie Bernard, Sumaira Kousar, Laurence Chevalier, et al.. Golgi-Mediated Synthesis and Secretion of Matrix Polysaccharides of the Primary Cell Wall of Higher Plants. Frontiers in Plant Science, 2012, 3, pp.79. ⟨10.3389/fpls.2012.00079⟩. ⟨hal-01846950⟩