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Journal articles
Tuning of Pectin Methylesterification: pectin methylesterase inhibitor 7 modulates the processive activity of co-expressed pectin methylesterase 3 in a ph-dependent manner
Abstract : Pectin methylesterases (PMEs) catalyze the demethylesterification of homogalacturonan domains of pectin in plant cell walls and are regulated by endogenous pectin methylesterase inhibitors (PMEIs). In Arabidopsis dark-grown hypocotyls, one PME (AtPME3) and one PMEI (AtPMEI7) were identified as potential interacting proteins. Using RT-quantitative PCR analysis and gene promoter::GUS fusions, we first showed that AtPME3 and AtPMEI7 genes had overlapping patterns of expression in etiolated hypocotyls. The two proteins were identified in hypocotyl cell wall extracts by proteomics. To investigate the potential interaction between AtPME3 and AtPMEI7, both proteins were expressed in a heterologous system and purified by affinity chromatography. The activity of recombinant AtPME3 was characterized on homogalacturonans (HGs) with distinct degrees/patterns of methylesterification. AtPME3 showed the highest activity at pH 7.5 on HG substrates with a degree of methylesterification between 60 and 80% and a random distribution of methyl esters. On the best HG substrate, AtPME3 generates long non-methylesterified stretches and leaves short highly methylesterified zones, indicating that it acts as a processive enzyme. The recombinant AtPMEI7 and AtPME3 interaction reduces the level of demethylesterification of the HG substrate but does not inhibit the processivity of the enzyme. These data suggest that the AtPME3·AtPMEI7 complex is not covalently linked and could, depending on the pH, be alternately formed and dissociated. Docking analysis indicated that the inhibition of AtPME3 could occur via the interaction of AtPMEI7 with a PME ligand-binding cleft structure. All of these data indicate that AtPME3 and AtPMEI7 could be partners involved in the fine tuning of HG methylesterification during plant development.
Cited literature [74 references]
https://hal-normandie-univ.archives-ouvertes.fr/hal-01841080
Contributor : Arnaud Lehner Connect in order to contact the contributor
Submitted on : Tuesday, July 17, 2018 - 9:28:15 AM
Last modification on : Friday, August 5, 2022 - 12:31:38 PM
Long-term archiving on: : Thursday, October 18, 2018 - 12:33:13 PM
Contributor : Arnaud Lehner Connect in order to contact the contributor
Submitted on : Tuesday, July 17, 2018 - 9:28:15 AM
Last modification on : Friday, August 5, 2022 - 12:31:38 PM
Long-term archiving on: : Thursday, October 18, 2018 - 12:33:13 PM
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2015-Senechal-et-al-J Biol Che...
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