HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

RING1B O-GlcNAcylation regulates gene targeting of polycomb repressive complex 1 in human embryonic stem cells

Abstract : O-linked-N-acetylglucosamine (O-GlcNAc) post-translationally modifies and regulates thousands of proteins involved in various cellular mechanisms. Recently, O-GlcNAc has been linked to human embryonic stem cells (hESC) differentiation, however the identity and function of O-GlcNAc proteins regulating hESC remain unknown. Here, we firstly identified O-GlcNAc modified human stem cell regulators such as hnRNP K, HP1γ, and especially RING1B/RNF2. Thereafter, we focused our work on RING1B which is the catalytic subunit of the polycomb repressive complex 1 (PRC1) a major epigenetic repressor essential for pluripotency maintenance and differentiation. By point-mutation, we show that T 250 /S 251 and S 278 RING1B residues are bearing O-GlcNAc, and that T 250 /S 251 O-GlcNAcylation decreases during differentiation. O-GlcNAc seems to regulate RING1B-DNA binding as suggested by our ChIP-sequencing results. Non-O-GlcNAcylated RING1B is found to be enriched near cell cycle genes whereas O-GlcNAcylated RING1B seems preferentially enriched near neuronal genes. Our data suggest that during hESC differentiation, the decrease of RING1B O-GlcNAcylation might enable PRC1 to switch its target to induce neuron
Complete list of metadata

Cited literature [26 references]  Display  Hide  Download

Contributor : Arnaud Lehner Connect in order to contact the contributor
Submitted on : Tuesday, July 17, 2018 - 9:19:09 AM
Last modification on : Wednesday, March 2, 2022 - 5:24:02 PM
Long-term archiving on: : Thursday, October 18, 2018 - 12:40:59 PM


2015-Maury-et-al-Stem Cell Res...
Publisher files allowed on an open archive



Julien Jean Pierre Maury, Chadi Farran, Daniel Ng, Yuin-Han Loh, Xuezhi Bi, et al.. RING1B O-GlcNAcylation regulates gene targeting of polycomb repressive complex 1 in human embryonic stem cells. Stem Cell Research, Elsevier, 2015, 15 (1), pp.182 - 189. ⟨10.1016/j.scr.2015.06.007⟩. ⟨hal-01841067⟩



Record views


Files downloads