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Analysis of Sugar Component of a Hot Water Extract from Arabidopsis thaliana Pollen Tubes Using GC-EI-MS

Abstract : Extraction with hot water is the oldest and simplest method used to recover pectin from an alcohol insoluble residue extract, although this method has not been widely used for the cell wall analysis of pollen tube, a model used to study cell wall. This protocol described this method applied for pectin extraction from 6 h-old Arabidopsis pollen tubes followed by a sugar composition analysis by gas chromatography mass spectrometry.
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Submitted on : Thursday, September 5, 2019 - 3:45:28 PM
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Marie Dumont, Arnaud Lehner, Corinne Loutelier-Bourhis, Jean-Claude Mollet, Patrice Lerouge. Analysis of Sugar Component of a Hot Water Extract from Arabidopsis thaliana Pollen Tubes Using GC-EI-MS. Bio-protocol , Bio-protocol LCC, 2015, 5 (12), pp.e1503. ⟨10.21769/BioProtoc.1503⟩. ⟨hal-01838553⟩

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