A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers

Marie Dumont (1) , Céline Cataye (2) , Arnaud Lehner (1) , Eric Maréchal (2) , Patrice P. Lerouge (1) , Denis Falconet (2) , Jean-Claude Mollet (1)

1 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2 LPCV - Laboratoire de physiologie cellulaire végétale

The pollen tube, a fast tip-growing cell, is an excellent model to study membrane and cell wall biosynthesis. Here, we describe a simple protocol using an easy to use device to perform immunofluorescence labelling of pollen tube membrane and cell wall. The use of the NucleoSpin column to perform all the steps of the immunolabelling procedure results in obtaining more intact pollen tubes.

Mots clés: Pollen tube; Cell wall; Membrane; Immunolabelling

Domaines: Polymères; Amélioration des plantes; Biochimie [q-bio.BM]; Biologie moléculaire; Génomique, Transcriptomique et Protéomique [q-bio.GN]; Réseaux moléculaires [q-bio.MN]; Phytopathologie et phytopharmacie; Biotechnologies; Gamétogenèse; Organisation et fonctions cellulaires [q-bio.SC]; Interactions cellulaires [q-bio.CB]

Marie Dumont, Céline Cataye, Arnaud Lehner, Eric Maréchal, Patrice P. Lerouge, et al.. A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers. Bio-protocol , 2015, 5 (12), pp.e1502. ⟨10.21769/BioProtoc.1502⟩. ⟨hal-01838518⟩