A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers

Marie Dumont (1) , Céline Cataye (2) , Arnaud Lehner (1) , Eric Maréchal (2) , Patrice P. Lerouge (1) , Denis Falconet (2) , Jean-Claude Mollet (1)

1 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2 LPCV - Laboratoire de physiologie cellulaire végétale

The pollen tube, a fast tip-growing cell, is an excellent model to study membrane and cell wall biosynthesis. Here, we describe a simple protocol using an easy to use device to perform immunofluorescence labelling of pollen tube membrane and cell wall. The use of the NucleoSpin column to perform all the steps of the immunolabelling procedure results in obtaining more intact pollen tubes.

Keywords: Pollen tube; Cell wall; Membrane; Immunolabelling

Domains: Chemical Sciences; Polymers; Life Sciences [q-bio]; Vegetal Biology; Plant breeding; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Biochemistry [q-bio.BM]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular biology; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Genomics [q-bio.GN]; Life Sciences [q-bio]; Biochemistry, Molecular Biology; Molecular Networks [q-bio.MN]; Life Sciences [q-bio]; Vegetal Biology; Phytopathology and phytopharmacy; Life Sciences [q-bio]; Biotechnology; Life Sciences [q-bio]; Development Biology; Gametogenesis; Life Sciences [q-bio]; Cellular Biology; Subcellular Processes [q-bio.SC]; Life Sciences [q-bio]; Cellular Biology; Cell Behavior [q-bio.CB]

Marie Dumont, Céline Cataye, Arnaud Lehner, Eric Maréchal, Patrice P. Lerouge, et al.. A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers. Bio-protocol , 2015, 5 (12), pp.e1502. ⟨10.21769/BioProtoc.1502⟩. ⟨hal-01838518⟩