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A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers

Abstract : The pollen tube, a fast tip-growing cell, is an excellent model to study membrane and cell wall biosynthesis. Here, we describe a simple protocol using an easy to use device to perform immunofluorescence labelling of pollen tube membrane and cell wall. The use of the NucleoSpin column to perform all the steps of the immunolabelling procedure results in obtaining more intact pollen tubes.
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Submitted on : Thursday, September 5, 2019 - 3:43:45 PM
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Marie Dumont, Céline Cataye, Arnaud Lehner, Eric Maréchal, Patrice Lerouge, et al.. A Simple Protocol for the Immunolabelling of Arabidopsis Pollen Tube Membranes and Cell Wall Polymers. Bio-protocol , Bio-protocol LCC, 2015, 5 (12), pp.e1502. ⟨10.21769/BioProtoc.1502⟩. ⟨hal-01838518⟩

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