Monoclonal Antibodies against Human Factor VIII Molecular Neutralize Antihemophilic Factor and Ristocetin Cofactor Activities.

Brigitte Sola (1) , Avner P (1) , Sultan Y (1) , Jeanneau C (1) , Maisonneuve P (1)

1 Inserm U152 - Immunologie et virologie des cancers. Immunologie des interactions cellulaires et moléculaires

A series of monoclonal antibodies have been raised against a preparation of the factor VIII/von Willebrand factor molecule. Of the seven hybridomas showing specific activity against the factor VIII molecule in a solid-phase radioimmunoassay, three (F4.55, F4.77, and F4.264) have been shown to partially inhibit ristocetin-induced platelet aggregation and two (F4.115 and F4.415) inhibit the antihemophilic activity of the factor VIII molecule. An additional monoclonal antibody was directed against a contaminant of the factor VIII preparation and is an antifibrinogen antibody.

Domaines: Sciences du Vivant [q-bio]

Brigitte Sola, Avner P, Sultan Y, Jeanneau C, Maisonneuve P. Monoclonal Antibodies against Human Factor VIII Molecular Neutralize Antihemophilic Factor and Ristocetin Cofactor Activities.. Proceedings of the National Academy of Sciences of the United States of America, 1982, ⟨10.1073/pnas.79.1.183⟩. ⟨hal-01762075⟩