Skip to Main content Skip to Navigation
Journal articles

Transcriptional and Post-Transcriptional Mechanisms Induce Cyclin-D1 over-Expression in B-Chronic Lymphoproliferative Disorders.

Abstract : Cyclin D1 participates in cell‐cycle control, in the progression through the G1 phase and in the transition from the G1 to the S phase. The CCND1 locus, located in 11q13, is amplified and cyclin‐D1 protein is over‐expressed in a wide range of human solid tumors. In some B‐lymphoid malignancies, the t(11;14)(q13;q32) translocation joins the Ig heavy‐chain locus to the CCND1 locus and leads to cyclin‐D1 over‐expression. In this study, a series of 127 patients presenting a B‐chronic lymphoproliferative disorder (B‐CLPD) was analyzed using a competitive RT‐PCR designed to detect cyclin‐D1‐mRNA over‐expression. Cyclin‐D1 mRNA was expressed in patients with mantle‐cell lymphoma (MCL; 10/10), hairy‐cell leukemia (HCL; 3/5), B‐chronic lymphoid leukemia (B‐CLL; 4/111) and B large‐cell lymphoma (BLCL; 1/1). Densitometric analysis of RT‐PCR products and Western‐blot autoradiograms, in addition to cytogenetic data, indicated that activation of the cyclin‐D1 gene occurred independently of the t(11;14)(q13;q32) translocation in patients with HCL. Indeed, a normal‐sized protein of 36 kDa exhibiting a level incompatible with gene activation by a translocation mechanism was detected in lymphoid cells with a normal karyotype. Moreover, we found a discrepancy between cyclin‐D1 mRNA and protein levels in MCL and B‐CLL, which suggested that some regulatory mechanisms acting at a post‐transcriptional level persist in tumor cells. Int. J. Cancer 83:230–234, 1999. © 1999 Wiley‐Liss, Inc.
Document type :
Journal articles
Complete list of metadatas

https://hal-normandie-univ.archives-ouvertes.fr/hal-01762045
Contributor : Université Normandie <>
Submitted on : Monday, April 9, 2018 - 3:52:42 PM
Last modification on : Wednesday, December 4, 2019 - 9:52:03 AM

Links full text

Identifiers

Collections

Citation

Brigitte Sola, Salaün V, Ballet Jj, Troussard X. Transcriptional and Post-Transcriptional Mechanisms Induce Cyclin-D1 over-Expression in B-Chronic Lymphoproliferative Disorders.. International Journal of Cancer, Wiley, 1999, 83, pp.230-234. ⟨10.1002/(sici)1097-0215(19991008)83:2<230::aid-ijc14>3.0.co;2-j⟩. ⟨hal-01762045⟩

Share

Metrics

Record views

81