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In Vitro Evaluation of B-CLL Cells Apoptotic Responses to Irradiation.

Abstract : Defective apoptosis is a mechanism which could possibly explain B chronic lymphocytic leukemia (B-CLL) cell accumulation. Differences in evolution and prognosis of B-CLL patients may be due to heterogeneity in apoptotic cell death. We studied the apoptotic response to in vitro gamma radiation of blood mononuclear cells from 18 untreated B-CLL patients. In cells irradiated with 2, 4 or 8 Gy and then cultured for 20 hours, the percentage of trypan blue excluding (viable) cells was not modified (>92%). An apoptotic response to irradiation was detected in the majority of the patients, but the individual percentage of apoptotic cells varied widely (8 to 81% after 8 Gy irradiation) in individual cases. The flow cytometric analysis of nick-end DNA labeling demonstrated a dose effect of irradiation, particularly in patients with an apoptotic response of over 20%. In the future, a valuable clue to the selection of irradiation regimens for B-CLL patients may be the investigation of correlations between in vitro radiation-induced apoptosis and the in vivo response to radiation therapy.
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Comby E, Andre I, Troussard X, Lebrun E, Brigitte Sola, et al.. In Vitro Evaluation of B-CLL Cells Apoptotic Responses to Irradiation.. Leukemia & lymphoma, Taylor & Francis, 1999, 34 (1-2), pp.159-166. ⟨10.3109/10428199909083392⟩. ⟨hal-01762044⟩



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