Ectopic Expression of Cyclin D1 Impairs the Proliferation and Enhances the Apoptosis of a Murine Lymphoid Cell Line

Duquesne F (1) , Florent M (1) , Roué G (1) , Troussard X (2) , Brigitte Sola (1)

1 Laboratoire de recherche Immunopathologie et infection
2 Laboratoire d'Hématologie Biologique [CHU Caen]

Cyclin D1, a key regulator of the cell cycle, acts as an oncogene when over-expressed in several types of cancer. In some B-chronic lymphoproliferative disorders, the over-expression of cyclin D1 protein is thought to confer a proliferative phenotype. We have generated BaF3 pro-B cell derivatives in which cyclin D1 can be induced rapidly and reversibly in a dose-dependent manner by the hormone muristerone A. When non-expressing clones displayed the same proliferative capacity as the parental cell line, in the sub-clones, a moderate induction of cyclin D1 lengthened the proliferation rate. The over-expression of cyclin D1 had the same effects on cell proliferation but also led ultimately to cell death by apoptosis. The induction of cyclin D1 in growth factor-deprived cells as well as in anticancer drug-treated cells also reinforced the magnitude of apoptosis. Thus, the expression of cyclin D1 in lymphoid cells does not confer a proliferative advantage but rather alters the response of cells towards apoptotic stimuli in a p53-independent manner. Cell Death and Differentiation (2001) 8, 51–62.

Domains: Life Sciences [q-bio]

Duquesne F, Florent M, Roué G, Troussard X, Brigitte Sola. Ectopic Expression of Cyclin D1 Impairs the Proliferation and Enhances the Apoptosis of a Murine Lymphoid Cell Line. Cell Death and Differentiation, 2001, 8, p. 51-62. ⟨10.1038/sj.cdd.4400768⟩. ⟨hal-01762037⟩