Skip to Main content Skip to Navigation
Journal articles

Intra-tracheal amikacin spray delivery in healthy mechanically ventilated piglets

Abstract : Background: Nebulization during mechanical ventilation is impeded by large extra-pulmonary drug deposition and long administration durations which currently limit implementation of inhaled antibiotic therapy. Direct intra-tracheal delivery using a sprayer represents an appealing alternative investigated in small animal models, but large animal data are lacking. Methods: Amikacin was administered through intravenous infusion (20 mg/kg), nebulization (60 mg/kg) and direct intra-tracheal spray (30 mg/kg) to 10 intubated piglets, in a randomized cross-over design. Amikacin concentrations were measured in the serum and pulmonary parenchyma. Anatomic deposition was investigated using immuno-histochemistry. Results: Spray delivery resulted in higher amikacin outputs than nebulization and infusion. Pulmonary inhaled delivery techniques yielded much higher lung concentrations and much lower serum concentrations than intravenous infusion. However, unlike nebulization and infusion, intra-tracheal spray delivery was associated with more than 100-and 1000-fold variability in lung concentrations between and within animals. Amikacin specific immuno-histochemistry showed consistent bronchial and alveolar drug deposition with all modalities. Conclusion: Nebulization remains the most reliable and simple technique to deliver inhaled amikacin uniformly to the lung during mechanical ventilation. Further development of tracheal sprays is required to take advantage of potential benefits related to high drug output and low extra-pulmonary deposition in large animals.
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-02526680
Contributor : Accord Elsevier Ccsd Connect in order to contact the contributor
Submitted on : Friday, October 22, 2021 - 10:07:00 PM
Last modification on : Thursday, November 25, 2021 - 3:43:51 AM

File

S1094553918301032.pdf
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial 4.0 International License

Identifiers

Citation

Antoine Guillon, François Darrouzain, Nathalie Heuzé-Vourc'H, Antoine Petitcollin, Céline Barc, et al.. Intra-tracheal amikacin spray delivery in healthy mechanically ventilated piglets. Pulmonary Pharmacology and Therapeutics, Elsevier, 2019, 57, pp.101807. ⟨10.1016/j.pupt.2019.101807⟩. ⟨hal-02526680⟩

Share

Metrics

Record views

138

Files downloads

91