HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Low effect of phenanthrene bioaccessibility on its biodegradation in diffusely contaminated soil

Abstract : This study focused on the role of bioaccessibility in the phenanthrene (PHE) biodegradation in diffusely contaminated soil, by combining chemical and microbiological approaches. First, we determined PHE dissipation rates and PHE sorption/desorption isotherms for two soils (PPY and Pv) presenting similar chronic PAH contamination, but different physico-chemical properties. Our results revealed that the PHE dissipation rate was significantly higher in the Pv soil compared to the PPY soil, while PHE sorption/desorption isotherms were similar. Interestingly, increases of PHE desorption and potentially of PHE bioaccessibility were observed for both soils when adding rhamnolipids (biosurfactants produced by Pseudomonas aeruginosa). Second, using C-13-PHE incubated in the same soils, we analyzed the PHE degrading bacterial communities. The combination of stable isotope probing (DNA-SIP) and 16S rRNA gene pyrosequencing revealed that Betaproteobacteria were the main PHE degraders in the Pv soil, while a higher bacterial diversity (Alpha-, Beta-, Gammaproteobacteria and Actinobacteria) was involved in PHE degradation in the PPY soil. The amendment of biosurfactants commonly used in biostimulation methods (i.e. rhamnolipids) to the two soils clearly modified the PHE sorption/desorption isotherms, but had no significant impact on PHE degradation rates and PHE-degraders identity. These results demonstrated that increasing the bioaccessibility of PHE has a low impact on its degradation and on the functional populations involved in this degradation.
Document type :
Journal articles
Complete list of metadata

https://hal.archives-ouvertes.fr/hal-01597866
Contributor : Archive Ouverte Prodinra Connect in order to contact the contributor
Submitted on : Friday, January 21, 2022 - 10:40:59 AM
Last modification on : Tuesday, May 10, 2022 - 3:38:05 PM
Long-term archiving on: : Friday, April 22, 2022 - 7:02:59 PM

File

Manuscript_Crampon et al_PrePr...
Files produced by the author(s)

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Citation

M. Crampon, A. Cébron, F. Portet-Koltalo, S. Uroz, F. Le Derf, et al.. Low effect of phenanthrene bioaccessibility on its biodegradation in diffusely contaminated soil. Environmental Pollution, Elsevier, 2017, 225, pp.663-673. ⟨10.1016/j.envpol.2017.03.053⟩. ⟨hal-01597866⟩

Share

Metrics

Record views

166

Files downloads

9